Definition
Isotope labeling can be used to track the changing rules of substances. This isotope is called tracer element. This element will not change the chemical nature of compounds. Scientists can know the chemical reaction with tracking the tracer element. This method is called isotope labeling.
Basic features
Radionuclide and their compounds, which are used during the process of isotope labeling have the same chemical nature and biological feature with the normal elements and their compounds, except for the nuclear physics properties. So scientists can use the isotope to be a marker and then they can produce the compounds with the isotope labeling to replace non-labeled compounds.
The advantages of stable isotope labeling
High sensitivity
Radioactive tracer method can track 10-14-10-18g. It is much more effective than weight analytical balance. And the most accurate chemical analysis in recent years can just only detect 10-12g.
Simple
Radioactive tracer method will not be affected by other non-radioactive substance. So many complicated steps can be ignored. When we are doing this experiment, we can get the result by detecting the x-ray released by some radioisotopes. So it has simplified the process of experiment.
Accurate position and quantification
Radioactive tracer method can detect the change and movement of some substances accurately with the help of some technologies. It can determine the distribution of radiotracers in tissues and organs.
Physiological conditions
The amount of radiolabeled compounds in the experiment of radioisotope is just a little. So that is to say it does not have an effect on the weight of material. Physiological processes still keep in a normal balance. So the result we got from the experiment can reflect the nature of some substance objectively.
Although radioactive tracer method has its own advantages, it has some disadvantages. The staff who is doing this experiment should have some experience of specialized training and they should protect them well during this process. What’s more, some elements do not have the suitable radioisotope, such as, oxygen, nitrogen. And we also have to pay more attention to effects of isotope tracer and radiation.
These are some basic knowledge about the stable isotope labeling and its advantages and some shortcomings. I hope this can give you more information on this aspect.
Isotope labeling can be used to track the changing rules of substances. This isotope is called tracer element. This element will not change the chemical nature of compounds. Scientists can know the chemical reaction with tracking the tracer element. This method is called isotope labeling.
Basic features
Radionuclide and their compounds, which are used during the process of isotope labeling have the same chemical nature and biological feature with the normal elements and their compounds, except for the nuclear physics properties. So scientists can use the isotope to be a marker and then they can produce the compounds with the isotope labeling to replace non-labeled compounds.
The advantages of stable isotope labeling
High sensitivity
Radioactive tracer method can track 10-14-10-18g. It is much more effective than weight analytical balance. And the most accurate chemical analysis in recent years can just only detect 10-12g.
Simple
Radioactive tracer method will not be affected by other non-radioactive substance. So many complicated steps can be ignored. When we are doing this experiment, we can get the result by detecting the x-ray released by some radioisotopes. So it has simplified the process of experiment.
Accurate position and quantification
Radioactive tracer method can detect the change and movement of some substances accurately with the help of some technologies. It can determine the distribution of radiotracers in tissues and organs.
Physiological conditions
The amount of radiolabeled compounds in the experiment of radioisotope is just a little. So that is to say it does not have an effect on the weight of material. Physiological processes still keep in a normal balance. So the result we got from the experiment can reflect the nature of some substance objectively.
Although radioactive tracer method has its own advantages, it has some disadvantages. The staff who is doing this experiment should have some experience of specialized training and they should protect them well during this process. What’s more, some elements do not have the suitable radioisotope, such as, oxygen, nitrogen. And we also have to pay more attention to effects of isotope tracer and radiation.
These are some basic knowledge about the stable isotope labeling and its advantages and some shortcomings. I hope this can give you more information on this aspect.