Summary
Itraq quantification is very useful for unbiased untargeted biomarker discovery. However, as the most widely used service, there are many questions about this Itraq proteomics service. Here I will list some common questions about this kind of service.
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The first question is what are the theory of the Itraq analysis and the advantage of it?
The theory of Itraq is the polypeptide markers and quantification. It can change the polypeptide into isotope of 114, 115, 116 and 117 to simplify the process of quantification. And then we will be back to the protein quantification through peptide quantification. The advantage of this service is that it can marker more than 8 samples at the same time with a few errors.
The second question is that is the Itraq proteomics service better than the 2-DGE service?
As the development of the technology of MALDI-TOF, 2-DGE service is getting on the stage. Technically, it can separate the protein according to distinguish of the IEF and SDS-PAGE. However, it cannot get the result. The Itraq analysis can overcome the disadvantage made by the 2-DGE service without the experiment of two-dimensional electrophoresis. It can detect about 2000 protein with only one needle. What’s more, it can detect more than 5 samples at the same time in order to reduce the error of instruments. In a word, the Itraq service is much more advanced than 2-DGE service.
The third question is that which one is the best for quantification among Itraq, SILAC and SWATH.
Among these methods for quantification, the Itraq is the most popular one. This service will not need samples to detect the quantification of protein. And after experiment, you can get the exact result. Although the label-free can reach this goal, the result cannot be promised. And SILAC is just suitable for the quantification of cells. SWATH can get the best result; however, the price is much higher than two services above.
About the author
Itraq mass spectrometry is also the hottest service in this field. According to the introduction above, we can find out that the Itraq proteomics is really important and useful.