Summary
Disulfide bond is very important in folding and stability in some proteins. It exists in all kinds of proteins, such as, hormones, enzymes, immunoglobulins of prokaryotic and eukaryotic creatures. As the development of technology in this field, there are many kinds of methods to be applied to the disulfide bond analysis. Here we will talk about the application of MS in disulfide bond analysis.
Body
At the beginning of the application of MS in the use of pepsase, which seems to be like diagonal electrophoresis. However, there is still a big difference between the two applications. The separation and analysis can be processed at the same time. Peptides, which have disulfide bond, can be detected by comparing the mass spectrogram of mixture of peptides in reductive alkylation. Edman can be used to determine the matching items disulfide bond. And then we can get the exact result.
Now many scientists have used the mass spectrometry method for the determination of the disulfide bond in the complex proteins. The modern biology mass spectrometry(MALDI-TOF-MS) has the advantage of little samples, the high-speed, the high tolerance of buffer solution and salt. The ESI-MS can be used with the liquid chromatography or capillary electrophoresis to quantify and determine the disulfide bond. What’s more, the Tetsuya can analyze the product of endoPG to determine the disulfide bond.
However, some times we have to determine some complex proteins by using the MS/MS. And according to the research, we can conclude that the complex peptides with the near cysteines can be determined with the help of MS/MS. It is also be useful in the disulfide bond prediction. The use of MS/MS can result in releasing more information of the disulfide bond determination. If we choose the pepsin to reduce the interaction of the disulfide bonds, this result will be influenced by its low specifity. But don’t be worried about that, we can take care of this by using the enzymolysis makers method.
About the author
Here I introduce the application of MS in disulfide bond analysis and its determination. If it is not complete, you can add by yourself and I think this will be informative for you.
Disulfide bond is very important in folding and stability in some proteins. It exists in all kinds of proteins, such as, hormones, enzymes, immunoglobulins of prokaryotic and eukaryotic creatures. As the development of technology in this field, there are many kinds of methods to be applied to the disulfide bond analysis. Here we will talk about the application of MS in disulfide bond analysis.
Body
At the beginning of the application of MS in the use of pepsase, which seems to be like diagonal electrophoresis. However, there is still a big difference between the two applications. The separation and analysis can be processed at the same time. Peptides, which have disulfide bond, can be detected by comparing the mass spectrogram of mixture of peptides in reductive alkylation. Edman can be used to determine the matching items disulfide bond. And then we can get the exact result.
Now many scientists have used the mass spectrometry method for the determination of the disulfide bond in the complex proteins. The modern biology mass spectrometry(MALDI-TOF-MS) has the advantage of little samples, the high-speed, the high tolerance of buffer solution and salt. The ESI-MS can be used with the liquid chromatography or capillary electrophoresis to quantify and determine the disulfide bond. What’s more, the Tetsuya can analyze the product of endoPG to determine the disulfide bond.
However, some times we have to determine some complex proteins by using the MS/MS. And according to the research, we can conclude that the complex peptides with the near cysteines can be determined with the help of MS/MS. It is also be useful in the disulfide bond prediction. The use of MS/MS can result in releasing more information of the disulfide bond determination. If we choose the pepsin to reduce the interaction of the disulfide bonds, this result will be influenced by its low specifity. But don’t be worried about that, we can take care of this by using the enzymolysis makers method.
About the author
Here I introduce the application of MS in disulfide bond analysis and its determination. If it is not complete, you can add by yourself and I think this will be informative for you.